Plate based translational impedance assays & MEA assays
Metrion currently has three multi-electrode array (MEA systems). Our systems enable stand-alone MEA recording (MED64, Axion Maestro) or MEA combined with impedance readout (CardioExcyte96). All three platforms offer plate-based recording at 37 °C using non-invasive, label free recording techniques for both short and long term monitoring of compound activity. Axion Maestro is a high throughput device containing a total of 768 electrodes and options to record in 12, 48 or 96-well formats. CardioExcyte96 offers dual readout from a single electrode in 96-well format, recording both electrical activity (MEA) and beating (impedance) from the same population of cells. MED64 offers stimulation via any two of its 64 electrodes and offers an impressive signal to noise ratio.
MEA is a powerful tool to investigate the electrophysiological characteristics of excitable cells. Human iPSC-derived cardiomyocytes plated directly on to recording electrodes rapidly establish a coordinated network of beating cells. Extracellular filed potentials (EFP) are then recorded and cell populations from different origins can be assessed for intrinsic activity or the effects of compounds on excitability, beat rate regularity and conduction velocity investigated. MEA recording can also be used to verify the actions of compounds developed to be active in a specific cell phenotype, e.g. atrial vs ventricular.
In confluent cell layers time-resolved impedance measurements track changes in cell shape and thus enables the quantitative monitoring of physical movement and adhesion of spontaneously, synchronously beating cardio myocytes.
Hybrid screening with CardioExcyte96 enables the integration of complementary information reflecting complex physiological parameters such as firing vs beat rate, amplitude and duration.
Recordings are made from cells within a network in a physiologically relevant environment making this platform ideal for investigating both acute short term exposure and chronic cardiotoxicity of compounds over a period of several days.