Central phenotypic assay platforms at Metrion include manual patch-clamp and multi-electrode array (MEA) techniques to establish compound target validation, target engagement and species selectivity. Alternatively, these methods can be used to electrophysiologically characterise cells with different etiology. Physiological activity is monitored from native tissue such as rodent cortical neurons (MEA burst & heat map shown) or other cell types such as those derived from stem cells.
Metrion utilise peripheral phenotypic assays to enable our customers to translate their compounds towards the clinic. We use manual patch-clamp and are developing multi-electrode array (MEA) techniques to establish target validation, target engagement and species selectivity. Physiological activity can be monitored from native tissue such as rodent dorsal root ganglia (DRG) or human DRG, as shown here.
Metrion has extensive experience of developing screening assays against specific endogenous ion channels expressed within native neurons to provide further compound validation. Determination of the pharmacology and selectivity of compounds in the intact cell milieu can help to bridge the gap between in vitro assays and in vivo applications. Assays can be designed specifically for your needs to explore the mechanism of action of compounds, so talk to us about your exact requirements.