Current-clamp recording

Current-clamp is an information rich technique which allows recordings from individual neurons to measure changes in membrane potential or firing behaviour in response to compound application or current input. Recordings can be used to compare firing characteristics from different cell types, verify findings from other sources (such as MEA) and to ascertain the mechanism of action (MOA) of compounds.

At Metrion, we are highly experienced at recording from native rodent neurons and are currently developing further translational assays such as recordings from stem cell derived neurons and human dorsal root ganglion neurons (see action potential data in panel).

Multi-electrode array

Multi-electrode arrays (MEA) enables simultaneous recording of the physiological activity in multiple peripheral neuronal cells. Extracellular field potentials are recorded in a non-invasive manner to characterise neuronal firing before and after compound addition. For example, these techniques can be used to examine cells in sensory pathways, such as rodent dorsal root ganglia neurons, and their response to ligand application (example shown). The electrophysiological behaviour of populations of disease modified neurons or cells from different sources can also be compared with relative ease.

Capsaicin Application