Native Neuronal Ion Channels
Metrion offers a set of reliable and reproducible neuronal ion channel assays to demonstrate the efficacy, selectivity, and potency of your therapeutic compounds.
Introduction to Native Neuronal Ion Channels
The gold standard of electrophysiological recording of ion channels is voltage-clamp using the manual patch clamp technique. Metrion are able to carry out recordings of neuronal ion channels from native rodent neurons and rodent and human neuroblastoma cell lines, and have the experience to be able to isolate the ion channel of interest, usingour extensive know-how of biophysics and pharmacology.
These assays often need to be specifically designed for your translational neuroscience and drug discovery needs, please contact us with your requirements using the buttons below.
Metrion’s experienced neuroscientists can devise studies using cultures of mixed populations or single cells, to enable comparisons of particular cell types and recording of isolated voltage- and ligand-gated ionic currents of interest. Such studies may be designed to extract in-depth biophysical data to allow a better understanding of the role of specific ion channels in generating neuronal excitability, and demonstrate the potential of client compounds to modulate such activity. An example of this approach is shown in Fig. 1 where voltage-gated properties of calcium currents are measured from rodent dorsal root ganglion neurons. Alternatively, the pharmacologicalinhibition or activation of specific ion channel subtypes by reference and test compounds can be examined in detail, helping to verify theirspecificity and mechanism of action (an important task as confirmation of published data is essential to give confidence during target i.d. and validation efforts). Our experienced scientists are adept at interpreting such data and making recommendations for next steps.
With the current focus on phenotypic screening using complex assay system, it is essential that the specific target(s) and signalling pathway(s) of active compounds are established to confirm their target specificity, selectivity and mechanism-of-action.As well as offering proven ion channel expertise, we have the right partners offering other techniques, and our neuroscience capabilities are growing and expanding all the time. Please talk to us about your exact requirements by contacting us using the various web forms.
Native Neuronal Ion Channel Resources
- Profiling endogenous sodium channels in the ND7-23 neuroblastoma cell line: implications for use as a heterologous ion channel expression system and native tissue model suitable for automated patch clamp screening. Royal Society for Chemistry Ion Channel Symposium, March 2016.
- Development of Native and Stem Cell-Derived Electrophysiological Assays for Neurotoxicology Screening and Translational Drug Discovery. SPS Berlin 2017 poster 142.
- Metrion Biosciences: high quality ion channel drug discovery service provider. Milner Therapeutics Symposium, Cambridge, 2019
- The role of Nav1.7 in human nociceptors: insights from human induced pluripotent stem cell-derived sensory neurons of erythromelalgia patients.
- Characterization of Endogenous Sodium Channels in the ND7-23 Neuroblastoma Cell Line: Implications for Use as a Heterologous Ion Channel Expression System Suitable for Automated Patch Clamp Screening.
- Voltage-clamp and current-clamp recordings from mammalian DRG neurons.
- Multiple sodium channels and their roles in electrogenesis within dorsal root ganglion neurons.
- The role of sodium channels in neuropathic pain.
- A single sodium channel mutation produces hyper- or hypoexcitability in different types of neurons.
- Marc Rogers (Metrion Director and CSO) outlines the benefits of targeting ion channels for pain and some of the hurdles in developing successful ion channel modulators.
- Marc Rogers (Metrion Director and CSO) takes part in a collaborative webinar with Nanion Technologies entitled “Validation and optimization of automated patch clamp voltage-gated Ca2+ channel assays”.
Native Neuronal Ion Channel Technologies
- QPatch automated electrophysiology
- Conventional manual patch clamp electrophysiology
Let’s work together
What are your specific ion channel screening requirements?
If you have any questions, or would like to discuss your project, we will put you directly in touch with a member of our scientific team. Contact us today to discover more.