Peripheral Neuronal Firing
Metrion offers a set of reliable and reproducible peripheral neuron assays to demonstrate the efficacy, selectivity, potency of your therapeutic compounds.
Introduction to Translational Peripheral Neuronal Assays and Platforms
Validating compounds in translational assays is vital to progressing your drug discovery program. Metrion offers a range of translational, phenotypic neuronal assays and platforms employing native rodent and human iPSCs from the peripheral nervous system. These assays are useful for determining the efficacy, potency and mechanism-of-action of client compounds designed to treat diseases such as pain and inflammation.
We mainly focus on electrophysiological readouts, using manual patch (voltage and current clamp) and multi-electrode array (MEA) platforms to record changes in single cell and neuronal network activity, and determine the effects of media, cell biology modulators, signalling pathways and test compounds.
These assays often need to be specifically designed for your translational neuroscience and drug discovery needs, please contact us with your requirements using the buttons below.
Current-clamp is an information rich technique which allows high fidelityrecordings from individual neurons to measure changes in membrane potential and firing behaviour in response to compound application or current input. Recordings can be used to compare firing characteristics from different cell types, verify findings from other sources (such as MEA) and to ascertain the mechanism of action (MOA) of compounds. In addition, we can make voltage clamp recordings of specific voltage- and ligand-gated ion channel currents to confirm the target specificity, selectivity and MoA of test compounds.
At Metrion, we are highly experienced at recording from native rodent neurons and are currently developing further translational assays such as recordings from stem cell derived neurons and human dorsal root ganglion neurons (see action potential data in panel).
Multi-electrode array (MEA) platforms enable simultaneous recording of the physiological activity in multiple peripheral neuronal cells. Metrion offer access to high fidelity single well MED64 and higher throughput Axion Maestro multiwell MEA systems for use with peripheral neuron preparations.Extracellular field potentials are recorded in a non-invasive manner to characterise neuronal firing before and after compound addition. For example, these techniques can be used to examine cells in sensory pathways, such as rodent dorsal root ganglia neurons, and their response to ligand application (Fig. 2). The electrophysiological behaviour of populations of disease-modified neurons or cells from different sources can also be compared with relative ease.
Peripheral Neuronal Firing Resources
- Development of Native and Stem Cell-Derived Electrophysiological Assays for Neurotoxicology Screening and Translational Drug Discovery. SPS Berlin 2017 poster 142.
- Profiling endogenous sodium channels in the ND7-23 neuroblastoma cell line: implications for use as a heterologous ion channel expression system and native tissue model suitable for automated patch clamp screening. Royal Society for Chemistry Ion Channel Symposium, March 2016.
- Metrion Biosciences: high quality ion channel drug discovery service provider. Milner Therapeutics Symposium, Cambridge, 2019
- The role of Nav1.7 in human nociceptors: insights from human induced pluripotent stem cell-derived sensory neurons of erythromelalgia patients.
- Characterization of Endogenous Sodium Channels in the ND7-23 Neuroblastoma Cell Line: Implications for Use as a Heterologous Ion Channel Expression System Suitable for Automated Patch Clamp Screening.
- Voltage-clamp and current-clamp recordings from mammalian DRG neurons.
- Multiple sodium channels and their roles in electrogenesis within dorsal root ganglion neurons.
- The role of sodium channels in neuropathic pain.
- A single sodium channel mutation produces hyper- or hypoexcitability in different types of neurons.
- Marc Rogers (Metrion Director and CSO) outlines the benefits of targeting ion channels for pain and some of the hurdles in developing successful ion channel modulators.
- Marc Rogers (Metrion Director and CSO) takes part in a collaborative webinar with Nanion Technologies entitled “Validation and optimization of automated patch clamp voltage-gated Ca2+ channel assays”.
Peripheral Neuronal Firing Technologies
- Conventional manual patch clamp electrophysiology
- Plate-based microelectrode array techniques
Let’s work together
What are your specific ion channel screening requirements?
If you have any questions, or would like to discuss your project, we will put you directly in touch with a member of our scientific team. Contact us today to discover more.